The Morphogenetic Effects of the Hooded Gene in Barley II. Cytological and Environmental Factors Affecting Gene Expression.

Effects of Treatment with Bone Morphogenetic Protein 4 and Co-culture on Expression of Piwil2 Gene in Mouse Differentiated Embryonic Stem Cells

Background Specific growth factors and feeder layers seem to have important roles in in vitro embryonic stem cells (ESCs) differentiation. In this study,the effects of bone morphogenetic protein 4 (BMP4) and mouse embryonic fibroblasts (MEFs) co-culture system on germ cell differentiation from mouse ESCs were studied. MaterialsAndMethods Cell suspension was prepared from one-day-old embryoid bo...

Validation of a genus-specific gene; TPS, used as internal control in quantitative Real Time PCR of transgenic cotton

Identification of genes with invariant levels of gene expression is a prerequisite for validating transcriptomic changes accompanying development. Ideally expression of these genes should be independent of the morphogenetic process or environmental condition.We report here the validation of internal control gene i.e.TPS (trehalose 6-phosphate-synthase) in cotton (Gossypium spp), using TaqMan sy...

Cloning and Expression of the Coat Protein Gene of Barley Yellow Dwarf Virus-PAV in Escherichia coli

Identification, Isolation and Expression Analysis of Hevein gene Family in Barley (Hordeum vulgar)

Today, antimicrobial peptides are known as a new generation of antibiotics for treatment of microbial diseases in human and animals and protecting plants against different pathogens. Heveins are a group of antimicrobial peptides which are considered as one of the most important groups of antimicrobial peptides due to the very high diversity and expression in different plant organs as well as th...

Effects of Oral Dosage of Lead Acetate II on Osteocalcin Gene Expression in Rat Mesenchymal Stem Cells

Background: Lead (Pb) is a heavy metal that has devastating effects on many animal tissues. In this study we investigated the effects of orally-dosed lead acetate II on osteocalcin gene (osteocalcin) expression in mesenchymal stem cells grown in an osteogenic medium. Osteocalcin is an abundant bone matrix differentiation protein. Methods: Twelve male Wistar rats were divided into three groups ...